10ml Shiitake Liquid Culture
This liquid culture can be inoculated on sterilised grains to make mother spawn. This species generates a heavy yield with many mushrooms. A combination of large and small cap mushrooms can be achieved. Stem length may vary depending on the carbon dioxide levels. Fruit body grows to have a soft cap with a chewy, succulent stem.
All our liquid cultures are made inside our sterile laboratory that is being filtered by a HEPA14 filter whilst maintaing a sterile technique. More than 99.995% of particles above 0.3 µm are filtered, ensuring a sterile environment. Your liquid culture will come in a sterile bag alongside a needle, that once opened should be used as soon as possible to decrease the risk of contaminants.
We aim to have all our liquid cultures ready to dispatch whenever there is an order, but because it is a growing mycelium sometimes dispatch may take longer. We will get in touch if this is the case.
Note: The described information has been tested thoroughly and the optimum conditions have been listed. Because of this, results may vary if the appropriate conditions are not met.
Instructions
Keep refrigerated at 2°C.
Take out of the fridge 24 hours before use for contents to reach room temperature.
Can be stored for up to 3 months at 2°C.
Cultivation Information
Species: Lentinula edodes
Recommended grain substrate: Oats, rye, wheat or similar
Recommended substrate: 80% Hardwood, 20% Bran or similar
Fruiting duration: 4-6 weeks*
Yield: Approx. 250-300g of fresh mushrooms per kg of our recommended wet substrate (1st flush); pre-inoculation
Inoculation % of wet substrate: 2-10%; higher inoculation % leads to a shorter incubation period. Do not exceed 10%.
*Please read induction information
Incubation Conditions
Room temperature: 20-22 °C
Substrate temperature: 22-24 °C
Duration: 13-15 days
Fruiting Conditions
Induction: To decrease the fruiting duration, submerge substrate block in cold water (<5°C) for 12 hours to shock primordia.
Optimum room conditions: 14-16 °C @ 85-95% RH
Carbon dioxide concentration: 400-600ppm
Light levels: 500-1000 lux
Flushes: 3-5 flushes @ approx 2 weeks interval with 85% RH, 1500-2500ppm CO2 concentration, temperature increase to 19-24 °C in between flushes
Bulk Orders
If you wish to purchase a large quantity of liquid cultures, please contact us.
Delivery Information
Please read the delivery information here to find information regarding dispatch times and more.
